Figure 5.

Preference for base-pair mismatches. (A) Five nanomolar Cy5-labeled dsDNA (45 bp), containing single base-pair mismatches (G/T, G/G, T/T, T/C, A/G, C/C, A/C, A/A), were incubated with various proteins as indicated on the panels. The products were separated by 10% PAGE. The representative results are shown. The base pairs are indicated on the top of each panel. The band assignments are indicated on the side of the panels, s, substrates; p, cleaved products. (B) Quantification of the cleaved products by 0.4 μM CglEndoMS and 0.2 μM Cglβ-clamp, and by 0.1 μM TkoEndoMS, as shown in lanes 4 and 7 in (A). The bars show the averages and the error bars are standard error of the mean (s.e.m.) from three independent experiments.