Table 1. ATPase kinetic rate constants of FANCJ wild-type and mutant proteins.

Protein	K m	k cat	k cat/Km
	(μM)a,b,c	(min-1)a,b,d	(min-1μM-1)
FANCJ-WT	505 ± 90	2730 ± 90	5.4
FANCJ-R707C	150 ± 50	921 ± 30	2.3
FANCJ-H396D	92 ± 30	339 ± 15	10

^aFANCJ concentrations were 60 nM.

^bM13mp18 ssDNA concentration was 2.1 nM.

^cATP concentration ranged from 30 μM to 8000 μM.

^dATP was 4.4 mM.