Figure 2.

mpk-1 mutation suppress csb-1 mutant sensitivity to transcription blocking lesions. (A) L1 larvae were treated 48 h with different concentrations of illudinM, in liquid culture, then plated and larval stages were determined immediately. (B) L1 larvae were treated 24 h with different concentrations of actinomycin D, in liquid culture, then plated and larval stages were determined 24 h later at 25°C. (C) L1 larvae were treated 24 h with different concentrations of α-amanitin, in liquid culture, then plated and larval stages were determined 24 h later at 25°C. Error bars represent standard deviation. *P < 0.05, **P < 0.01, ***P < 0.001, two-tailed t-test comparing the fraction of each larval stage of two different mutants for the same treatment condition. mpk-1(ga111);csb-1 and mpk-1(sbj10);csb-1 were compared to csb-1. Statistical analysis of additional comparison and Fisher’s Exact test for analyzing the distribution of larval stages see Supplementary Tables S2 and 8. Each treatment was conducted in triplicate with a minimum of 30 animals for each sample. Representative of three different experiments shown.