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Primer Extension Assays with template dC and incoming dGTP, dGMPnPP and dGMPPnP. The tested dPols added dGTP and could not add the α-β modified dGMPnPP opposite template dC. The β-γ modified dGMPPnP was also not incorporated into the primer and therefore, hydrolysis of PPi is important for completion of the DNA synthesis reaction.
