Table 1. Results of MA assay.
| Strain | WT | ΔendoMS | dnaE (D647G) | ΔendoMS-dnaE (D647G) | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Number of lines | 37 | 20 | 16 | 16 | ||||||||||
| Generation per line | 2034 | 174 | 174 | 124 | ||||||||||
| Mutation types | Number of mutations* | % of each mutation | Number of mutations* | % of each mutation | Number of mutations* | % of each mutation | Number of mutations* | % of each mutation | ||||||
| Transition | A:T→G:C | A→G | 48 | 61 | 18.1 | 137 | 236 | 25.8 | 76 | 112 | 13.2 | 626 | 1400 | 22.7 |
| T→C | 13 | 4.9 | 99 | 18.7 | 36 | 6.3 | 774 | 28 | ||||||
| G:C→A:T | G→A | 40 | 107 | 15.1 | 105 | 290 | 19.8 | 61 | 195 | 10.6 | 643 | 1149 | 23.3 | |
| C→T | 67 | 25.3 | 185 | 34.9 | 134 | 23.3 | 506 | 18.3 | ||||||
| Transversion | A:T→C:G | A→C | 11 | 27 | 4.2 | 1 | 3 | 0.2 | 36 | 68 | 6.3 | 22 | 42 | 0.8 |
| T→G | 16 | 6 | 2 | 0.4 | 32 | 5.6 | 20 | 0.7 | ||||||
| G:C→T:A | G→T | 11 | 27 | 4.2 | 0 | 1 | 0 | 28 | 69 | 4.9 | 19 | 51 | 0.7 | |
| C→A | 16 | 6 | 1 | 0.2 | 41 | 7.1 | 32 | 1.2 | ||||||
| A:T→T:A | A→T | 2 | 7 | 0.8 | 0 | 0 | 0 | 10 | 15 | 1.7 | 9 | 19 | 0.3 | |
| T→A | 5 | 1.9 | 0 | 0 | 5 | 0.9 | 10 | 0.4 | ||||||
| G:C→C:G | G→C | 5 | 11 | 1.9 | 0 | 0 | 0 | 1 | 5 | 0.2 | 9 | 16 | 0.3 | |
| C→G | 6 | 2.3 | 0 | 0 | 4 | 0.7 | 7 | 0.3 | ||||||
| Indels | Deletion | 14 | 5.3 | 0 | 0 | 45 | 7.8 | 37 | 1.3 | |||||
| Insertion | 11 | 4.2 | 0 | 0 | 65 | 11.3 | 48 | 1.7 | ||||||
| Overall | 265 | 530 | 574 | 2762 | ||||||||||
*Mutations are represented as that occurred in leading strand.