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. 2018 Jan 19;27(7):1174–1185. doi: 10.1093/hmg/ddy032

Figure 2.

Figure 2.

Damage of the basement membrane and disorganization of glial cells underlie cerebral and cerebellar malformations in Nestin-fukutin-cKO mice. (A) (Left column) Immunofluorescence analysis of the developing cortex at E18.5. Radial glia fibers were disorganized and extended into subarachnoid spaces (white arrow) through breaches in the basement membrane (asterisk) in cKO mice. In contrast, they had merged properly in littermate controls. Nestin and laminin were used as markers of radial glia or the basement membrane, respectively. (Right column) H&E staining of serial sections. Ectopic cells were observed at cerebral fissures (black arrow) in cKO mice. (B) Immunofluorescence analysis of the cerebellum at P7. Between adjacent cerebellar lobules, the basement membrane was not detected (asterisk), and many ectopic cells were observed (arrow) in cKO mice. Bergmann glia fibers were disorganized, and reactive gliosis was detected in such regions. In contrast, they had merged properly in littermate controls. GFAP was used as a marker of Bergmann glia. Scale bars = (A, B) 50 μm.