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Citrus leaf companion cell protoplast viewed under different light sources and filters after incubation in 0.15 μM Hoechst 3342, 100 nM MitoTracker, and 80 μM Neutral Red for 15 min. Each cell was viewed under brightfield microscopy (A); fluorescence microscopy (Rhodamine filter; Ex: BP 545/25, Em: BP 525/50) for red and green fluorescence (B); and under DAPI filter (Hoechst 3342; Ex: G365, Em: BP 445/50) for blue fluorescence (C).
