Fig.5.

Interactions between Aβ₄₂ and Bri2 BRICHOS. PLA signals (red) for protein interactions with (A,B) anti-Bri2 linker and anti-Aβ₄₂ antibodies; (D,E) anti-Bri2 BRICHOS and anti-Aβ₄₂ antibodies. Staining of nuclei is performed with DAPI (blue) and filamentous actin is stained with phalloidin (green). Scale bar 20 μm. (C,F) Fluorescence intensity quantification for negative controls (black bars) and Bri2 linker plus Aβ₄₂ (hatched bar), n = 4 (C), and negative controls and Bri2 BRICHOS plus Aβ₄₂ (hatched bar), n = 3 (D). ns, p > 0.05 and.**p < 0.01. Average values and errors represent standard deviations. The results are representative of at least three independent experiments.