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. 2017 Aug 2;31(11):5102–5110. doi: 10.1096/fj.201700463R

Figure 2.

Figure 2.

Confocal microscopy of P. aeruginosa biofilms. GFP-tagged PA01 biofilms at 24 and 48 h in absence and presence of rMIF. Using biofilm flow cell system, GFP-tagged PA01 was allowed to form biofilms for 24 and 48 h at 37°C in absence or presence of 100 ng/ml rMIF. biofilms were then imaged using confocal microscope to collect z-stack compositions. Images were analyzed by Imaris software(Bitplane, Belfast, Ireland). A) Representative image of biofilms grown for 24 h in LB medium only. Scale bar, 50 μm. B) Representative image of biofilms grown in presence of 100 ng/ml rMIF for 24 h. Scale bars, 50 μm. C) Significant increase in volumes of biofilms grown for 24 h, in presence of 100 ng/ml rMIF, when calculated by Imaris software. Data are presented as means ± sem cubic micrometers (n = 10). A Mann-Whitney U test was used to analyze data. ***P < 0.001. D) At 48 h, significant increase in volume of PA01 biofilms grown in presence of 100 ng/ml rMIF was demonstrated, compared to LB alone, when calculated by Imaris software. Data are presented as means ± sem cubic micrometers (n = 10). A Mann-Whitney test was used to analyze data. ***P < 0.001.