Figure 3.

rMIF increases survival of (A) PA01 and (B) PA14 after treatment with tobramycin. Biofilms were grown for 24 h, with or without 100 ng/ml MIF, and then treated with 50 µg/ml of tobramycin for a further 24 h. Biofilms were sonicated, serially diluted, and plated on agar to analyze CFU per milliliter as measure of bacterial survival. A) PA01 demonstrates significant increase in bacterial survival when it is grown in presence of MIF 100 ng/ml before treatment with 50 µg/ml of tobramycin. Data are presented as means ± sem CFU per milliliter (N = 10). B) Biofilms of PA14 have significantly increased survival against tobramycin when pretreated with 100 ng/ml of rMIF compared to control grown in LB alone. Data are presented as means ± sem CFU per milliliter (n = 4). *P < 0.05, **P < 0.01; Kruskal-Wallis with Dunn post hoc test.