Figure 5.

Significant decrease in percentage weight change of mice on d 7 and presence of neutrophils after infection and treatment with SCD-19 in murine model of PA pulmonary infection. Animals were injected 30 min before intratracheal instillation and daily thereafter with 35 mg/kg SCD-19 (solubilized in 5% DMSO) or as indicated. Body weight was measured to monitor extent of infection. A) Day 7 weights are expressed as percentage of start weight. PA-infected, PA + DMSO, and PA + ISO-1 groups all lost significant amount of weight compared to uninfected control group; however, SCD-19 group had not lost significant amount of weight at end of study. Data are presented as means ± sem of percentage of body weight (n = 6 per group). A 1-way ANOVA with post hoc Tukey-Kramer test was used to test for statistical differences. *P < 0.05, **P < 0.01. B) Differential cell counts were performed on BAL fluid from right lung of mice. Significant decrease in percentage of neutrophils in mice treated with SCD-19 was found in comparison to PA-infected mice. Neutrophil numbers were assessed by differential cell count using Diff-Quick stain and are expressed as means ± sem of percentage total cell count. A Kruskal-Wallis test with Dunn’s multiple comparison post hoc test was used to test for statistical differences (n = 6 per group). ***P < 0.01.