Figure 2.

Siglec-6 engagement inhibits IgE-mediated human MC activation. Primary human MC were primed with human IgE overnight, and then incubated in the absence or presence of mouse IgG (isotype) or mouse anti-human Siglec-6 (Siglec6). Subsequently, cells were exposed to control medium or various concentrations of anti-human IgE (α hIgE). Supernatants were collected after 90 min to measure beta-hexosaminidase (β-hex) (A). The purified subset of MC was selected for analysis of CD63 expression (B). Supernatants were collected after 16 h to measure GM-CSF (C) and IL-8 (D). ^*P < 0.05; ^**P < 0.005; ^***P < 0.001; ^****P < 0.0001, two-way ANOVA. Values are mean ± SEM of 3 independent experiments.