Figure 3.

Siglec-6 is upregulated on colon cancer-cocultured MC. (A) Representative histograms of surface Siglec-6 on primary human MC cocultured with or without colon cell lines (CCD841, Caco2, and HT29). (B) MFI of surface Siglec-6 was compared on primary human MC cocultured with vs. without colon cell lines. (C) Immunoblotting of Siglec-6 in colon cell lines and primary human MC cocultured with or without colon cell lines. Tryptase was used as an internal control and quantification of Siglec-6 was normalized to tryptase density. ^****P < 0.0001, one-way ANOVA. Data are representative of 2–5 independent experiments.