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. 2018 Sep 20;9:2150. doi: 10.3389/fimmu.2018.02150

Figure 1.

Figure 1

B cell development in St6gal1-KO mice. St6gal1-KO (KO) mice were backcrossed onto a C57BL/6J (WT) background for 15 generations to control for strain-specific differences. (A) Schematic of B cell development from the immature to mature stages in the bone marrow and spleen, as proposed by Carsetti and colleagues. (B) Frequency of B cells in the bone marrow (upper panel) and B cell subpopulations (lower panel) in WT and KO mice (n = 5). (C) Splenic mass and cell counts in WT and KO mice (upper panels). Frequencies of splenic B cell subpopulations in WT and KO mice (lower panel; n = 10). (D) Hematoxylin and eosin-stained spleens, with location of relevant anatomical compartments (WP, white pulp; RP, red pulp; MZ, marginal zone). Immunofluorescence microscopy of B220 (red) and marginal zone marker MARCO (green). (E) Mean fluorescence intensity of cell surface CD19, CD24, IgM, and CD23 in IgM-high bone marrow B cells, with FSC and SSC of gated cells shown (n = 5). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.