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. 2018 Sep 20;9:2150. doi: 10.3389/fimmu.2018.02150

Figure 6.

Figure 6

Extrinsic sialylation promotes transitional B cell development and CD23 expression. Wild-type immature B cells were cultured for 40 h with or without recombinant ST6Gal-1. Phenotypically B220-low (IM), IgM-hi (T), and B220-high (M) B cells are designated. In all panels, absence or presence of recombinant ST6Gal-1 (rST6G) in culture is indicated with “−” or “+.” (A) Mean fluorescence intensity for SNA is shown (n = 7). (B) Relative size of B cell populations after culture period (n = 7). (C) Expression of cell surface IgM and CD23 in the transitional population before culture, and after culture with or without extrinsic sialylation (n = 3). Results are representative of 3 independent experiments. *P < 0.05, **P < 0.01, ****P < 0.0001.