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The cotransfection assay. Cultured cells are transfected with the receptor cDNA in an expression vector (the trans-vector). The function of this transcription factor can be monitored by the activity of a reporter gene (the luciferase gene) linked to an appropriate hormone-responsive promoter. In this case, the promoter is from the MMTV virus carrying a GRE enhancer. The trans-vector encodes the hGR, shown combining with the steroid hormone (triangle).
