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RXRα present in liver nuclear extracts enhances PPAR binding to the PPRE. Gel mobility shift assays were prepared using 32P-labelled PPRE oligonucleotide and in vitro- synthesized PPAR and/or RXRα and 0.5 μg of rat liver nuclear extract (NE) as indicated. Pre-immune (PI) or polyclonal antiserum prepared against RXRα (RXRab) was included in the reactions.
METHODS. Gel mobility shift assays were done as described for Fig. 2.
