Figure 10. Sequence Comparison of the Direct Repeat HREs.
(a) rOST, the rat osteocalcin gene promoter (−455 to −441 from a transcription start site; Demay et al., 1990); hOST, the human osteocalcin gene promoter (−499 to −485; Ozono et al., 1991); mSPP-1, the mouse Sppllosteopontin promoter (−757 to −743; Noda et al., 1990). We confirmed that this spp-l VDREconfers vitamin D3 response io the basal reporter ΔSV-CAT, but a similar motif found in the 3’ end of the mouse laminin Bl RARE (mLamB1, −448 to −433 shown as an antisense after Vasios et al., 1989) does not function as a VDRE (data not shown).
(b) rMHC, the rat cardiac MHC α gene promoter (−149 to −134; Izumo and Mahdavi, 1988; Glass et al., 1989); hMHC, the human MHC promoter (−158 to −143; Flink and Morkin, 1990); rME, the rat malic enzyme gene promoter (−276 to −261; Petty et al., 1990); MLV, positions between 334 and 350 shown as an antisense (Sap et al., 1989); rS14, the rat S14 gene TRE (personal communication from How ard C. Towle). The oligonucleotides encoding DR-4M (A toT change in the 5’ end of each of the half-sites in DR-4) or the mouse skeletat actin gene promoter upstream sequence (mACTIN, −597 to −582 from a transcription start site; Hu et al., 1986) failed to confer T3 response to the basal reporter (data not shown). For rGH21 and βRARE-1, see text.
(c) mRARβ, the mouse RAR type β gene promoter (Sucov et al., 1990); hRARβ, the human RAR type β gene promoter (−52 to −37; de The et al., 1990); mCP-H, the mouse complement factor H gene promoter (−143 to −127; Muiioz-Cdnoves et al., 1990); hADH3, the human alcohol dehydrogenase gene promoter (−300 to −284 shown as an antisense; Duester et al., 1991). Oligonucleotides coding for a motif found in 2.4 kb upstream of the human muscle creatine kinase gene (hMCK; Trask et al., 1988) did not confer RA response (data not shown).