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. Author manuscript; available in PMC: 2018 Sep 27.
Published in final edited form as: Circ Res. 2011 Mar 17;108(9):1102–1111. doi: 10.1161/CIRCRESAHA.110.229393

Figure 1. Activation of the AngII/AT1r signaling pathway increases vascular calpain activity.

Figure 1

Calpain activity in vascular mesenteric extracts was measured using a fluorescent based assay with the use of the fluorescent probe T- Succ-LLVY-AMC. Two-week infusion of subpressor doses of AngII to rats (20 ng/Kg/min; left panel) or mice (500 ng/Kg/min; right panel) significantly increases calpain activity. A 5-day treatment of rat and mice with 10 mg/kg/day of the AT1r blocker losartan prevents upregulation of calpain activity in response to AngII. Similar results were obtained following treatment of AngII infused rats with two different calpain inhibitors, 140 μg/kg/day ZLLal or 1 mg/kg PD105. AngII failed to increase calpain activity in the vasculature of mice deficient in the AT1r. Data are expressed as the mean±SEM. Numbers at the base of the bars indicate the number of animals studied in each group.