Figure 4. Functional circuitry is altered in old mice and in APPSwInd mice.

(A) Representative examples of individual raw images for young WT, young APPSwInd, old WT and old APPSwInd mice (top four images), and within-group averaged images from young WT, young APPSwInd, old WT and old APPSwInd mice (bottom four images). Images are from the 24h post-injection time point. White arrows, injection site; red arrowheads, septum. Also see Supplemental Figure 1.

(B) Statistical parametric maps generated by automated, unbiased, voxel-wise whole brain pairwise within group t-tests are projected onto a grayscale image of our 3D mouse brain template atlas. Selected slices from 3D images show statistically significant Mn²⁺-enhanced intensity at 24h compared to 0.5h for each genotype in coronal (left panel), sagittal and axial slices (right panel) as indicated: young WT (green), young APPSwInd (yellow), old WT (blue), and old APPSwInd (red). Injection site is on the mouse's right, left side of the images. Arrowheads indicate contralateral hippocampus. Arrows point to septal regions. Viewing planes of these slices are indicated by blue lines on the 3D mouse brain template atlas shown in the bottom left panel. Note the robust transport in the young mice, and the altered location of the most significant voxels in the old WT and old APPSwInd, p<0.01 FDR corr.