Figure 4.

¹H-NMR spectroscopy defines T-like conformation of an engineered insulin monomer. Solution structure of DKP-insulin highlighting the aromatic triplet (Phe^B24, Phe^B25 and Tyr^B26) in brown relative to Val^B12, Leu^B15 and cysteine B19-A20 in blue. In this monomeric analog dimerization was impaired by the paired substitutions Lys^B28 and Pro^B29 [57], and the trimer interface was disrupted by Asp^B10 [58, 59]. The A chain is shown in yellow, and B chain in orange (B1-B19) and brown (B20-B30).