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Allergic sensitization and challenge protocol. Mice were sensitized (day 1, 3, and 5) and challenged (day 12-14) intranasally with PBS (30 μl/day; delivery vehicle; white triangles), PM (33.3 μg/day, light grey triangles), OVA (10 μg/day, dark grey triangles) or OVA+PM. Mice were euthanized 24 hr after the final challenge (day 15) to assess pulmonary inflammation.
