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. 2018 Aug 7;128(10):4573–4587. doi: 10.1172/JCI121957

Figure 7. Transcriptional alteration of global B cell subsets by CHB infection.

Figure 7

(A) Four different mature (CD19+CD10–) B cell populations from 5 CHB patients (CHB; eAg+CInf) and 4 healthy vaccinated subjects were FACS sorted based on their expression of CD21 and CD27 (see gating strategy). Cells were lysed, and mRNA expression levels of 588 immune-related genes were measured by NanoString. Heatmaps showing immune genes that are significantly different between CHB and healthy vaccinated subjects (P < 0.05, ≥2-fold different) within the 4 different B cell subsets, naive, RM, AM, and AtM. (B) Four different subsets of global CD4+ and CD8+ T cells (CD3+) were sorted from 6 CHB patients and 5 healthy vaccinated controls based on their expression of CCR7 and CD45RA (see gating strategy) and analyzed by NanoString. EM, effector memory; CM, central memory. Heatmaps showing immune genes that are significantly different between CHB and healthy vaccinated subjects (P < 0.05, ≥2-fold different) within the different T cell subsets.