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. 2018 Jul 26;128(10):4329–4342. doi: 10.1172/JCI99315

Figure 4. EC-Cd36–/– improves glucose homeostasis and increases insulin sensitivity in 6-month-old male mice fed a HFD for 4 months.

Figure 4

(A–E) BW, plasma lipids, and glucose after a 16-hour fast. (F) Plasma insulin after a 16-hour fast followed by a 4-hour refeeding period. P value was determined by 1-way ANOVA. (G) For GTTs, male mice fasted for 16 hours were given oral glucose, and tail blood samples were collected for glucose measurements at 0, 15, 30, 60, and 120 minutes. Also shown is the AUC of glucose excursions. (H) For ITTs, mice fasted for 4 hours were given 0.75 U/kg BW insulin i.p. Tail blood samples were obtained at t0 and then 15, 30, 45, 60 and 120 minutes after insulin administration. (I) Activity of IR, AKT, and S6 in Cd36fl/fl and EC-Cd36–/– mice 20 minutes after insulin injection, with densitometric analysis of (I) heart, (J) liver, and (K) skeletal muscle tissue. (L and M) Individual ceramide (Cer) species in heart (n = 5) and muscle (n = 9–10). Data represent the mean ± SD. *P < 0.05 and #P < 0.01 compared with Cd36fl/fl controls; 1-way ANOVA with Dunnett’s multiple comparisons test (A–E and G–M).