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. 2018 Jul 26;128(10):4329–4342. doi: 10.1172/JCI99315

Figure 5. EC-Cd36–/– mice have reduced tissue LCFA uptake during fasting.

Figure 5

(A–D) Uptake of oleic and hexanoic acids in EC-Cd36–/– mice. Four-month-old mice were fasted for sixteen hours and given a tail i.v. injection of [3H]oleic acid and [14C]hexanoic acid. (A) Plasma 3H radioactivity in Cd36fl/fl, EC-Cd36–/–, and CM-Cd36–/– mice (n = 5–6) measured at the indicated time points after injection. Scale bars: 50 μm. (B) 3H content of different tissues, (C) plasma 14C radioactivity, and (D) 14C content of different tissues. (E) Real-time [11C]palmitic acid uptake into heart, and (F) tissue [11C]palmitic acid uptake in Cd36fl/fl and EC–Cd36–/– male mice (n = 4). (G) Tissue FDG uptake in Cd36fl/fl and EC–Cd36–/– mice (n = 4) derived from summing the uptake kinetics up to 60 minutes after tracer administration. Also shown are representative scans of FDG accumulation at 60 minutes in the hearts of control and EC-Cd36–/– 16-hour–fasted mice. Max, maximum. Data represent the mean ± SD. *P < 0.05, #P < 0.01, and §P < 0.001 compared with Cd36fl/fl controls; 1-way ANOVA with Dunnett’s multiple comparisons test.