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. 2018 Sep 17;128(10):4543–4556. doi: 10.1172/JCI120912

Figure 2. PKM2 is crucial for EC proliferation and migration in vitro.

Figure 2

(A) Western blot analysis of PKM2 and PKM1 protein expression in HUVECs demonstrates that the siRNAs used in this study give PKM2-specific and efficient knockdown. qPCR analysis of PKM2 and PKM1 mRNA expression. Note that the y axis for PKM2 is a log scale (n = 3). (C) Growth curve of HUVECs with various siRNAs targeting PKM2. The efficiency of knockdown shown in B correlates with the effect on proliferation (n = 5). (D) Percentage of BrdU+ cells is reduced in siPKM2 ECs (n = 3). (E) Apoptotic cell death, assessed by Annexin V and PI staining, is not induced in siPKM2 ECs (n = 3). (F) Reduction in transwell migration of ECs by siPKM2. Cells that migrated across the transwell membrane were visualized by staining with phalloidin (red) and DAPI (blue) (n = 3). (G) Scratch closure was retarded in ECs with siPKM2 (n = 3). (H) Tube formation on Matrigel was impaired in ECs with siPKM2 (n = 3). Scale bars, 100 μm. All data are mean ± SD. **P < 0.01, by 2-tailed Student’s t test.