Skip to main content
. 2018 Sep 17;128(10):4543–4556. doi: 10.1172/JCI120912

Figure 5. Pyruvate kinase activity of PKM2 is dispensable for EC proliferation.

Figure 5

(A) Western blot and pyruvate kinase activity analysis of empty vector (E), PKM1 (1), or PKM2 (2) overexpression in the presence of siPKM in HUVECs (n = 3). (B) Growth curve demonstrating complete or no rescue of proliferation with PKM2 or PKM1 overexpression, respectively, in the presence of siPKM (n = 4). (C) Pyruvate kinase activity assay and growth curve in the presence or absence of 10 μM PKM2 activator (TEPP-46) (n = 4). (D) Western blot and pyruvate kinase activity analysis of empty vector (E), PKM2 WT (2), or PKM2 PEP binding mutant (K270M) overexpression in the presence of siPKM in HUVECs (n = 3). (E) Growth curve demonstrating complete rescue of proliferation with PKM2 PEP binding mutant (K270M) overexpression (n = 4). All data are mean ± SD. **P < 0.01, by 2-tailed Student’s t test.