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. 2018 Jul 17;128(10):4260–4279. doi: 10.1172/JCI97117

Figure 11. Resistance is by avoidance of selinexor-induced nuclear relocation of mutant NPM1/PU.1. OCI-AML3 NPM1-mutated cells were selected for resistance to selinexor by culture in selinexor, with up to 50 nM added every 3 days.

Figure 11

(A) NPM1 and PU.1 localization in the resistant cells by IF. DAPI was used to stain for nuclei. Images by Leica SP8 inverted confocal microscope; magnification, ×630. (B) NPM1 and PU.1 cytoplasmic versus nuclear localization in the resistant cells. WB of nuclear and cytoplasmic fractions. (C) Parental and selinexor-resistant OCI-AML3 cells were sensitive to noncytotoxic concentrations of decitabine. Cell counts by automated counter. Mean ± SD for 3 independent experiments. *P < 0.01 (significant after Bonferroni’s correction), selinexor or decitabine versus vehicle on day 7, 2-sided t tests.