Figure 5. Compound 3 regulates immune signaling.

(A) Heatmap showing differentially expressed genes in OCI-Ly3 cells treated for 8 or 24 hours with compound 3 relative to their vehicle controls. Highlighted are genes for relevant significantly enriched pathways: Roquin targets, JAK/STAT pathway, or MHC class II [MHCII]) genes. (B) GSEA for Roquin targets in OCI-Ly3 and TMD8 at 8 and 24 hours. cpd, compound; veh, vehicle. (C) Heatmap showing statistically significant pathways enriched in the upregulated or downregulated genes 24 hours after compound 3 treatment in OCI-Ly3 cells by hypergeometric test. PID, Pathway Interaction Database. (D) Western blot for p-STAT3 (Y705) in cell extracts of OCI-Ly3 and TMD8 treated for 8 or 24 hours with 100 nM or 1 μM compound 3. Results are representative of 2 experiments. (E) Heatmap of MHC class II genes showing log₂ FC expression of compound 3–treated cells versus vehicle-treated cells for OCI-Ly3 and TMD8 at 8 and 24 hours. (F) Schematic representation of the changes in mRNA expression for compound 3–regulated genes and their relationship to MALT1.