Skip to main content
. 2018 Aug 2;128(10):4525–4542. doi: 10.1172/JCI120401

Figure 6. RNF8 mediates negative regulation of Notch signaling in human breast cancer.

Figure 6

(A) RT-qPCR analysis of the fold change in the expression of Notch targets in the indicated RNF8-knockdown (shRNF8) human breast cancer cell lines compared with their controls (shCtr). Dot plots show mean ± SEM of 3 independent experiments. *P < 0.05, ***P < 0.001, ****P < 0.0001, 1-way ANOVA followed by Tukey’s test. (B) Immunoblot analysis of the indicated proteins in MDA-MB-231 cells transfected with FLAG empty vector (–) or RNF8-FLAG. (C) Immunoblot analysis of cytoplasmic (CE) and nuclear (NE) extracts from indicated cells. (D) Supervised clustering (from low to high RNF8 expression) of the profiles of genes with a significant negative or positive correlation (Pearson’s correlation coefficients [PCCs]) with RNF8 in the TCGA breast cancer (BRCA) data set. The breast cancer subtypes based on PAM50 are indicated. Data in B and C are representative of at least 3 experiments.