Figure 7. REVERBa regulates lipid metabolism.

C57BL/6 (WT) and REVERBaKO mice were given 1 mg/kg i.p. dex or vehicle at ZT6 every 48 hours for 8 weeks. Mice were assessed for effects on lipid metabolism. Body weight (A) and body fat percentage by EchoMRI (B) were measured at the start and end of the study (n = 7–8). Visceral fat adipocyte size was assessed by H&E (C) and quantified (D) (x axis values, U/μm²; n = 5, average of 3 fields); original magnification, ×10. (E) H&E of liver, collected at cull; original magnification, ×10. Liver triglycerides (F), serum triglycerides (TG) (G), and free fatty acids (FFA) (H) were also analyzed (n = 8 liver, n = 7–8 serum). Graphs show data for individual animals with median; adipocyte area shows individual values and mean. Statistical analysis via 2-way ANOVA repeated measures (body weight and fat mass), 2-way ANOVA and 3-way ANOVA (adipocyte size), or Kruskal-Wallis test with a Dunn’s multiple comparison correction (hepatic triglycerides, serum triglycerides, and serum free fatty acids), *P < 0.05, **P < 0.01, ****P < 0.0001.