Fig 4.

Screening for the expression of chimeric antibodies in A. thaliana seeds using SDS-PAGE (A, C) and western blot (B, D). The expression of chimeric antibodies in seeds was confirmed for extracts positive in ELISA. Western blots were developed with a mouse anti-histidine tag monoclonal antibody and a goat anti-mouse antibody conjugated to HRP. As negative controls, extract of wild-type seeds and the V1G chimeric antibody lacking the His-tag [23] were used. A His-tagged nanobody was used as a positive control, resulting in a protein band with a molecular weight of approximately 15 kDa. (A) IgA chimeric antibodies with V1, Nb23 and Nb5, (C) IgY chimeric antibodies with V1, Nb23 and Nb5.