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. 2018 Sep 27;13(9):e0204222. doi: 10.1371/journal.pone.0204222

Fig 10. Transient expression of anti-flagellin chimeric antibodies in N. benthamiana leaves and the interaction with purified flagellins and motile C. jejuni KC40.

Fig 10

(A) ELISA for the detection of chimeric antibodies in serial dilutions (1/4–1/8192) of extracts of infiltrated leaves. (B) Western blot results of leaf extracts with Nb2Flag8, Nb2Flag24 and Nb2Flag67 nanobodies fused to the Fc-domain of chicken IgA. The western blot was developed with anti-IgA conjugated to HRP. As a negative control, leaf extract of wild-type N. benthamiana was used. (C) Binding curve of twofold serial dilutions (1/4–1/8192) of leaf extract with coated purified flagellins. (D) Binding curve of twofold serial dilutions (undiluted– 1/2048) of leaf extract with coated C. jejuni KC40 bacteria. Anti-IgA conjugated to HRP was used for the development of the ELISA.