Table 1. The pEAQ-HT-DEST1 and pPhasGW expression plasmids encoding nanobodies fused to the constant domains of IgA or IgY.
Plasmid | Characteristics |
---|---|
pEAQ-HT | pEAQspecialK with CPMV-HT cassette [43] |
pEAQ-HT-DEST1 | Gateway-compatible pEAQ-HT destination vector [43] |
pGV5689 | pEAQ-HT-DEST1 + Nb5-IgY |
pGV5679 | pEAQ-HT-DEST1 + Nb5-IgA |
pGV5923 | pEAQ-HT-DEST1 + Nb2Flag8-IgA |
pGV5925 | pEAQ-HT-DEST1 + Nb2Flag24-IgA |
pGV5927 | pEAQ-HT-DEST1 + Nb2Flag67-IgA |
pPhasGW | Gateway-compatible vector [47] |
pGV5774 | pPhasGW + V1-IgY |
pGV5768 | pPhasGW + V1-IgA |
pGV5772 | pPhasGW + Nb5-IgY |
pGV5778 | pPhasGW + Nb5-IgA |
pGV5776 | pPhasGW + Nb23-IgY |
pGV5770 | pPhasGW + Nb23-IgA |