Fig 4. Western blot of recombinant S. pseudintermedius wild-type and attenuated LukS-I and LukF-I with HRP-conjugated anti-6xhis tag monoclonal antibody.

The Molecular weights of LukS-I, LukF-I, attenuated LukS-I and attenuated LukF-I determined in western blots using pre-stained Protein Standard (x 1,000) were of the expected sizes 39.43, 39.12, 37.27 and 37.59 kDa, respectively.