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. 2018 Sep 27;13(9):e0204450. doi: 10.1371/journal.pone.0204450

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© 2018 Abouelkhair et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 5 — Antibodies against S. pseudintermedius wild-type LukS-I and LukF-I were detected using an indirect ELISA. Recombinant S. pseudintermedius LukS-I and LukF-I proteins were coated on ELISA plates, then incubated with two-fold serially diluted serum from dog vaccinated with the same proteins. High reactivity with LukS-I and LukF-I was seen from sera collected two weeks after 3^rd injetions of attenuated LukS-I (P <0.0001****) and LukF-I (P <0.0001****) compared to pre-injection sera. The values represent averages from three independent experiments.