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. 2018 Sep 27;13(9):e0204450. doi: 10.1371/journal.pone.0204450

Fig 6. Cytotoxic effect of S. pseudintermedius recombinant Luk-I on canine PMNs.

Fig 6

PMN permeability assay using Sytox Green to detect the cytotoxic effect of wild-type Luk-I on canine PMNs. (A) Luk-I significantly induced PMN killing after 30 min compared to that with wild-type LukS-I (P = 0.0330***) and LukF-I (P = 0.0468**). (B) A1:2 S. pseudintermedius 06–3228 supernatant dilution significantly induced PMN killing after 30 min compared to that with and attenuated LukS-I (P = 0.0276**), attenuated LukF-I (P = 0.0268**) and attenuated Luk-I treatments (P = 0.0044***). The mean fluorescent intensity (MFI) of all treatment were calculated based on average values from three independent experiments. (*P < 0.05 was considered significant). ns–Not significant.