Table 2. Primers used in this study to amplify recombinant wild type and attenuated LukS-I and LukF-I from Staphylococcus pseudintermedius.

LukS-I-forward	GCATGAGGATCCGGTAAAAAATAAATTATTAGCCGCAACA
LukS-I-reverse	GCATGAGCGGCCGCATTATGCCCCTTTACTTTAATTTCGTG
LukS-I-M forward	GCATGAGGATCCAAGGCCACGTGTCTTGTC
LukS-I-M reverse	GCATGAGCGGCCGCCCCATGAGGCCAGTCTTG
LukF-I-forward	GCATGACTCGAGAAAAGAATGGCTAATCAAATTACACCTGTATCTG
LukF-I-reverse	GCATGAGGATCCTTAGTGATGGTGATGGTGATGTACTGTATGCTGATCCCAATCAA
LukF-I-M forward	GCATGAGGATCCGGATCCAATGAAAATAAGCAAAGTTATC
LukF-I-M reverse	GCATGAGCGGCCGCGCGGCCGCTGATGGGTTTTTT

NotI, XhoI and BamHI restriction sites are underlined.