Table 3. Plasmids and competent cells used to clone and express recombinant wild type and attenuated Staphylococcus pseudintermedius LukS-I and LukF-I.

Plasmid/ Bacteria	Expressed Gene	Source
pMA-LukS-I-M	Contain attenuated S. pseudintermedius LukS-I (LukS-I-M)	Synthetic gene, Life Technologies Corp., Carlsbad, CA
pMA-LukF-I-M	Contain attenuated S. pseudintermedius LukF-I (LukF-I-M)	Synthetic gene, Life Technologies Corp., Carlsbad, CA
pETBlue-2	LukS-I and LukS-I-M and LukF-I-M expression with blue/white screening and C-terminal HSV and His tagged sequences	Novagen, Madison, WI
Dh5-alpha (DE3) pLacI	Cloning and recombinant LukS-I and LukS-I-M and LukF-I-M protein expression	Novagen, Madison, WI
pKLAC2	An integrative expression vector of S. pseudintermedius LukF-I in yeast	New England Biolabs, Ipswich, MA
Kluyveromyces lactis	An expression host of S. pseudintermedius LukF-I	New England Biolabs, Ipswich, MA