Table 1. Primer sequences, GenBank accession numbers and expected amplicon sizes for qRT-PCR analyses.
| Target genes | Primers | Sequences (5' to 3') | GenBank accession number |
Product size (bp) |
|---|---|---|---|---|
| Oct4 | Forward | CGCAACGAGAGGATTTTGAG | TC_168415 | 68 |
| Reverse | CGCCAGAGGAAAGGATACTG | |||
| Nanog | Forward | TGCCTGGTGAACGCTTCTGGAA | NM_001129971 | 104 |
| Reverse | TGGTTGCTCCAAGACTGGCTGT | |||
| Sox2 | Forward | AGAAGAACAGCCCAGACCGAGTT | NM_001123197 | 108 |
| Reverse | CCGAGTTGTGCATCTTGGGGTT | |||
| Cdx2 | Forward | CCTCTCGCCCACAAATGTTCAC | TC_206866 | 82 |
| Reverse | TCCAACCGCACCTGTCTTTACC | |||
| Bcl-xL | Forward | GCAGGTATTGAACGAACTCTTCCG | AJ_001203 | 107 |
| Reverse | GCATCTCCTTGTCTACGCTCTCC | |||
| Bax | Forward | CTACCAAGAAGTTGAGCGAGTGTC | AJ_606301 | 85 |
| Reverse | ACGGCTGCGATCATCCTCTG | |||
| β-actin | Forward | CCACGCCATCCTGCGTCT | AK_237086 | 168 |
| Reverse | CCATCTCCTGCTCGAAGTCCAG |