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Dependency of CaMan5_18 activity with pNP-βMan as substrate on (A) temperature, and on (B) pH. (C) Kinetic stability of CaMan5_18 hydrolysis of pNP-βMan. Symbols: filled circles, 40°C; empty circles, 50°C; filled triangles, 60°C; empty triangles, 70°C. (D) ThermoFluor analysis of thermal stability to unfolding as a function of pH.
