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Separation of the hydrolysis products from time-dependent hydrolysis of mannooligosaccharides and Man-GlcNAc by wild-type CaMan5_18. (A) M3 and M4; (B) M6 and M5 with the mannooligosaccharide standards M1-M6 at the left; (C) Man-GlcNAc hydrolysis by CaMan5_18 wild type and the E140Q/E259Q double mutant.
