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. 2018 Sep 21;11:6153–6165. doi: 10.2147/OTT.S175428

Figure 2.

Figure 2

Effects of CPEB4 silencing or overexpression on GC cells’ migration, invasion, and proliferation in vitro.

Notes: (A) Detection of relative expression of CPEB4 protein in GC cell lines and normal gastric epithelial GES-1 cells by Western blot. (B and C) Successful knockdown of CPEB4 in SGC7901 cells and CPEB4 overexpression in AGS cells were confirmed by Western blot and immunofluorescence. Magnification ×400. (D and E) The effects of CPEB4 expression on the migration and invasion ability of SGC7901 and AGS cells were determined by wound-healing assay and transwell assay. The effects of CPEB4 expression on the proliferation ability of SGC7901 and AGS cells were measured by the CCK-8 assay. Magnification ×100. (F), colony formation assay (G), and EdU assay (H). *P<0.05. Magnification ×200.

Abbreviations: CCK-8, cell-counting kit-8; CPEB4, cytoplasmic polyadenylation element-binding protein 4; GC, gastric cancer.