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. 2018 Sep 21;11:6153–6165. doi: 10.2147/OTT.S175428

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© 2018 Cao et al. This work is published and licensed by Dove Medical Press Limited

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Effects of different expression levels of CPEB4 on EMT-related markers in SGC7901 and AGS cells.

Notes: Following CPEB4 shRNA or overexpression treatment, qRT-PCR (A), Western blot (B), and immunofluorescence (C) were used to determine the expressions of EMT-related markers in CPEB4-altered cells, including the epithelial marker E-cadherin, the mesenchymal markers N-cadherin and Vimentin, and transcription factors (Snail, Slug, ZEB1, SIP1, and Twist). ^*P<0.05. Magnification ×400.

Abbreviations: CPEB4, cytoplasmic polyadenylation element-binding protein 4; EMT, epithelial–mesenchymal transition; qRT-PCR, quantitative real-time PCR; shRNA, short hairpin RNA.