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. 2018 Sep 20;11:1523–1536. doi: 10.2147/IDR.S173455

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© 2018 López-García et al. This work is published and licensed by Dove Medical Press Limited.

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Detection of the OprD protein in 17 clinical bla_IMP-carrying P. aeruginosa. (A, C, E) PAGE-SDS of extract total protein, (B, D, F) Western blot of extract total protein with polyclonal antisera anti-oprD.

Notes: For each lane, 30 μg of proteins were loaded. P. aeruginosa PAO1 and OprD protein recombinant were used as positive controls. (A, B) Line 1: Molecular weight marker (Spectra^tm); Line 2: recombinant OprD protein; Line 3: P. aeruginosa PAO1; Line 4: PE06 (stop codon); Line 5: PE09 (L7-short); Line 6: PE15 (not amplified); Line 7: PE16 (stop codon), Line 8: PE20 (IS1394); Line 9: PE21 (stop codon). (C, D) Line 1: Size marker; Line 2: recombinant OprD protein; Line 3: P. aeruginosa PAO1; Line 4: PE22 (L7-short); Line 5: PE23 (stop codon); Line 6: PE27 (partially amplified), Line 7: PE46 (L7-short); Line 8: PE52 (stop codon); Line 9: PE62 (stop codon); Line 10: PE63 (wild type). (E, F) Line 1: Size marker; Line 2: recombinant OprD protein; Line 3 P. aeruginosa PAO1; Line 4: PE64 (stop codon); Line 5: PE73 (stop codon); Line 6: PE83 (stop codon) and Line 7: PE10 (L7-short). The experiment was made in duplicate.