Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Sep 20;11:1523–1536. doi: 10.2147/IDR.S173455

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2018 López-García et al. This work is published and licensed by Dove Medical Press Limited.

The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.

PMC Copyright notice

Detection of bla_IMP in plasmids of eight P. aeruginosa strains by Southern blot.

Notes: (A) PFGE after digestion with nuclease S1 (180 U/μL, Takara Bio Inc.), (B) Southern hybridization of plasmids (DIG HIGH Prime DNA Labeling and Detection Starter Kit II) using bla_IMP as a probe. Line 1: Control strain E. coli NCTC 50192 with four plasmids: 154 Kb, 66 Kb, 48 Kb and 7 Kb, Line 2: PE06; Line 3: PE21; Line 4: PE52; Line 5: PE62; Line 6: PE63; Line 7: PE64; Line 8: PE73 and Line 9: PE83. The black arrows indicate the positive signal corresponding to the hybridization with bla_IMP in plasmids. The experiment was done three times.

Abbreviation: PFGE-S1, Pulsed-field gel electrophoresis with nuclease S1.