Table 1.
Phenotype and genotype of resistance of P. aeruginosa clinical isolates carrying blaIMP gene
| Strain | PFGE type | Phenotype of resistance |
aMIC (μg/mL)
|
Genotype | Amino acid changes
|
|||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CAZ | FEP | MEM (+ bEPI) | IMP | CIP (+ bEPI) | AN | GM | GyrA | ParC | ||||
| PE06 | 15 | PIP, TIC, TZP, CAZ, FEP, ATM, IPM, MEM, GM, NN, AN, CIP, NOR | >512 | >512 | >512 (>512) | >512 | >512 (>512) | >512 | 32 | blaIMP-18, blaIMP-56, blaOXA-2, blaOXA-50, aadA1, aacA6 | T83I | S87L |
| PE09 | 6a | TIC, CAZ, FEP, IPM, MEM, GM, NN, AN, CIP, NOR | >512 | 512 | >512 (32) | 64 | 64 (32) | 256 | >512 | blaIMP-18, blaOXA-2, blaOXA-50, aadA4 | T83I | S87L |
| PE10 | 8 | PIP, TIC, CAZ, FEP, ATM, IPM, MEM, GM, NN, AN, CIP, NOR | 512 | 256 | 64 (64) | 64 | 32 (16) | 64 | >512 | blaIMP-18, blaOXA-2, blaOXA-50, aadA4 | T83I | S87L |
| PE15 | 7 | TIC, CAZ, FEP, IPM, MEM, GM, NN, AN, CIP, NOR | 512 | 256 | 64 (64) | >512 | 32 (16) | 64 | >512 | blaIMP-18, blaOXA-2, blaOXA-50, aadA4 | T83I | S87L |
| PE16 | 6b | TIC, CAZ, FEP, IPM, MEM, GM, NN, AN, CIP, NOR | 512 | 256 | 64 (64) | >512 | 32 (16) | 64 | >512 | blaIMP-18, blaOXA-2, blaOXA-50, aadA4 | T83I | S87L |
| PE20 | 1 | PIP, TIC, CAZ, FEP, IPM, MEM, GM, NN, AN, CIP, NOR | >512 | >512 | >512 (128) | >512 | 128 (64) | >512 | >512 | blaIMP-18, blaOXA-2, blaOXA-50, aadA4 | T83I | S87L |
| PE21 | 28 | TIC, CAZ, FEP, IPM, MEM, GM, NN, AN, CIP, NOR | >512 | 512 | >512 (>512) | 128 | 128 (64) | >512 | >512 | blaIMP-62, blaGES-32, blaOXA-2, blaOXA-50, aacA33, aadA1, aadA6, aacA4, aacA7 | T83I | S87L |
| PE22 | 12 | TIC, CAZ, FEP, IPM, MEM, GM, NN, AN, CIP, NOR | 512 | 256 | 16 (16) | 64 | 128 (64) | 128 | >512 | blaIMP-18, blaOXA-2, blaOXA-50, aac(3′)-IIa, aadA4 | T83I | S87L |
| PE23 | 10 | TIC, FEP, IPM, MEM, GM, NN, AN, CIP, NOR | 16 | 32 | 32 (32) | 64 | 32 (16) | 64 | >512 | blaIMP-62, blaGES-2, blaOXA-2, blaOXA-50, aacA4, aacA29b | T83I | S87L |
| PE27 | 25 | TIC, CAZ, FEP, IPM, MEM, GM, NN, AN, CIP, NOR | 512 | 256 | 64 (64) | >512 | 64 (32) | 128 | 512 | blaIMP-62, blaGES-2, blaOXA-2, blaOXA-50, aacA4 | T83I | S87L |
| PE46 | 32 | TIC, CAZ, FEP, IPM, MEM, GM, NN, AN, CIP, NOR | >512 | 512 | >512 (32) | 64 | 64 (32) | 512 | >512 | blaIMP-18, blaGES-2, blaOXA-2, blaOXA-50, aacA4 | T83I | S87L |
| PE52 | 16 | PIP, TIC, TZP, CAZ, FEP, ATM, IPM, MEM, GM, AN, CIP, NOR | >512 | >512 | >512 (>512) | >512 | >512 (512) | >512 | 32 | blaIMP-18, blaIMP-56, blaOXA-2, blaOXA-50, aadA1, aacA6 | T83I | wild type |
| PE62 | 20 | PIP, TIC, CAZ, FEP, ATM, IPM, MEM, GM, NN, AN, CIP, NOR | >512 | >512 | >512 (>512) | >512 | >512 (512) | >512 | 16 | blaIMP-18, blaOXA-2, blaOXA-50, | T83I | S87L |
| PE63 | 38 | PIP, TIC, TZP, CAZ, FEP, ATM, IPM, MEM, GM, NN, AN, CIP, NOR | 256 | 232 | 512 (512) | 512 | 128 (64) | 512 | >512 | blaIMP-18, blaGES-1, blaOXA-2, blaOXA-50, aacA4, aadA1 | wild | P105T |
| PE64 | 21 | PIP, TIC, CAZ, FEP, ATM, IPM, MEM, GM, NN, AN, CIP, NOR | 256 | 512 | >512 (512) | >512 | 512 (256) | >512 | 32 | blaIMP-18, blaOXA-2, blaOXA-50 | T83I | S87L |
| PE73 | 26 | PIP, TIC, CAZ, FEP, ATM, IPM, MEM, GM, NN, AN, CIP, NOR | >512 | >512 | >512 (>512) | >512 | >512 (512) | >512 | 32 | blaIMP-56, blaOXA-2, blaOXA-50 | T83I | S87L |
| PE83 | 13 | TIC, CAZ, FEP, IPM, MEM, GM, NN, AN, CIP, NOR | >512 | >512 | >512 (>512) | 64 | 256 (128) | >512 | >512 | blaIMP-62, blaGES-26, blaOXA-2, blaOXA-50, aac(3′)-IIa, aacA4, aadA6, aacA7 | T83I | S87L |
Notes:
a
Minimum Inhibitory Concentration;
b
PAβN (L-phenylalanine-arginine-β-naphthylamide).
Abbreviations: PFGE, pulsed-field gel electrophoresis; PIP, piperacillin; TIC, ticarcillin; TZP, tazobactam; CAZ, ceftazidime; FEP, cefepime; IMP, imipenem; MER, meropenem; GM, gentamicin; NN, tobramycin; AN, amikacin; CIP, ciprofloxacin; NOR, norfloxacin; ATM, aztreonam.