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Effect of mechanical stretch on expression of BDNF mRNA in rat colonic circular SMC in vitro. Primary culture of rat colonic circular SMCs were treated without (empty bar) or with static stretch (filled bar) at different elongation (5%, 10%, and 18%) for 1 hr, and cells were harvested 3 hours after stretch ended. Total RNA was extracted for qPCR quantification of BDNF mRNA expression. N = 5 or 6 independent experiments, * p < 0.05 vs no-stretch (N.S.) control.
