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. Author manuscript; available in PMC: 2019 Sep 26.
Published in final edited form as: Cell Syst. 2018 Aug 1;7(3):323–338.e6. doi: 10.1016/j.cels.2018.06.010

Figure 5. ST SseC interacts with the retromer complex in vivo and in vitro.

Figure 5

(A) Highest ranking genes that specifically correlate with ST SseC, with gene #19 included to depict all five components of the retromer complex (underlined). (B) Distribution of all ST SseC S-scores and z-scores with the five retromer mutants represented as red circles. (C) Immunoblot analysis of interaction between 3xFLAG-ST SseC and VPS35 (HA-tagged and endogenous), HA-VPS26A, HA-TBC1D5. (D) In vitro pull-down assays between ST SseC and single retromer components (VPS26A, VPS29, and VPS35) (left panel) or combinations of retromer components (right panel). SscA, a chaperone for SseC (Cooper et al., 2013), was included in all reactions. CTL (control) (E) Equilibrium binding of ST SseC with combination of retromer components by SPR. The dissociation constant (Kd) was derived by fitting of the equilibrium binding data to a one site binding model (right panel).