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Fluorescent intensity per RBC versus mass of sulfo-NHS-biotin per mL of RBC for BioRBC-6, -18, -54, and -162. The least square regression plots for these in vitro mixtures of BioRBC prepared using the three wash buffers and isotonic saline are nearly superimposable. Fluorescein-conjugated avidin was used as the fluorescent stain (ImmunoPure® Avidin, Fluorescein Conjugated, cat#21221, Thermo Scientific, Rockford, IL). Data from Figure 2.
